Fig. 4
From: ISO-FLUCS: symmetrization of optofluidic manipulations in quasi-isothermal micro-environments
ISO-FLUCS reduces the temperature impact while still inducing net thermoviscous flows. a, e Maximum intensity projection over time showing the particle trajectories obtained by repeated scanning along a single line (a, highlighted in pink) and along seven parallel lines (e, the lines highlighted in orange are directionally counteracted, while the middle line in pink is always scanned right to left). b, f Histograms of the particle velocity measured within the cyan dashed regions highlighted in panels a and e, respectively, by the TrackMate plugin implemented in the Fiji software. c, g Temperature distributions measured from Rhodamine B dispersed in glycerol/water solution by using the scan patterns in a and e, respectively. d, h Corresponding histograms of the temperature measured within the yellow dashed region. i–l Particle trajectories, histogram of the particle velocity, temperature distribution, and histogram of the temperature, respectively, measured with the ISO-FLUCS pattern shown in panel e by doubling the laser power. Scale bar: 20 μm