Fig. 2
From: ISO-FLUCS: symmetrization of optofluidic manipulations in quasi-isothermal micro-environments
Semi-empirical simulations to determine the scan pattern with minimal temperature impact. a Time-averaged fluorescence image of Rhodamine B when exposed to the IR laser. The red dashed rectangle highlights the region of interest (ROI) where the intensity cross-section was evaluated. b Temperature profile of a heat spot measured from Rhodamine B fluorescence in glycerol/water solution. The data are best fitted by a Cauchy-Lorentz curve (Eq. 1). c Simulated temperature distribution induced by a spiral pattern and d corresponding temperature histogram. e Simulated temperature distributions induced by two heat spots placed at different separation distances. The corresponding intensity cross-sections (solid white curves) reveal a relatively homogeneous temperature profile within the ROI (yellow dashed rectangle) at approximately 5.6-μm separation distance. f Standard deviation of the temperature distribution measured within the ROI in panel e as a function of the separation distance between the heat spots indicates an optimal distance of 5.6 μm. g Simulated temperature distribution induced by a spiral ISO-FLUCS pattern and h corresponding temperature histogram