Fig. 5
From: Structured illumination microscopy based on principal component analysis
Real-time super-resolution reconstructions of mitochondria of live COS-7 cells labeled by MitoTracker\(^{TM}\) Green FM at different time points. a, d Comparison of wide-field images and super-resolution images obtained by PCA-SIM. Wide-field images are displayed in the bottom left triangle, which were obtained by averaging 9 raw SIM images (with the resolution of \(512 \times 512\)) captured through a 60\(\times\) objective (UPlanXApo 60\(\times\)/1.42 Oil, Olympus, Japan). Super-resolution images reconstructed by PCA-SIM are shown on the upper right. b, e Magnified super-resolution images from the blue boxed regions in a and d at different time points. c, f Magnified super-resolution images from the white boxed regions in a and d at different time points. Colored arrows point to regions where dynamics are distinct. Scale bars: 5 \(\mu\)m (a), (d); 1 \(\mu\)m (b) left, (c), (e) and (f); 0.5 \(\mu\)m (b) right