Fig. 1

TIS window for bilateral cortical observation post-TBI. a Fluorescence imaging of a TBI mouse on a centimeter scale using a commercial fluorescence magnification microscope. The purple asterisk indicates the central location of the TBI. b–d Larger views of the three areas demarcated with box lines in (a). e–g The results of neutrophil and vessel segmentations in (b–d), respectively. h–j Tracked neutrophils in each ROI. The colors represent the average velocities of the various neutrophils. k-l The relationship between the travel distance and the distance to k the injury center or l the midline. m–n The relationship between the travel velocity and the distance to m the injury center or (n) the midline. o–r Quantitative analyses of neutrophil cytodynamics in three ROIs. Travel distance: the length of the path taken by a trajectory; Travel velocity: travel distance divided by the time of movement; Travel displacement: distance between the end point and start point of each track; Travel speed: travel displacement divided by the time of movement. s Fluorescence imaging of another TBI mouse showing bleeding across several hours of observation. The purple asterisk indicates the central location of the TBI. The red dotted box highlights the bleeding area. t Enlarged view of the area in the solid blue line box in (s). u Changes in the average velocity of neutrophils in the area during observation. t1 denotes when the bleeding started. t2 indicates when the bleeding spread to the area. The red zone marks a sudden acceleration in bleeding