Fig. 5

Simulation results of 2p-MINFLUX. a Configuration of a simulated single-color dye-labelled square DNA origami. (b-d) Simulation on the above origami of b 2p-MINFLUX and c, d 1p-MINFLUX. For each dye, simulation of localization was repeated for b, d 25 and c 100 times respectively, with N = 250 (b, c) or 1000 (d), L = 50 nm, and SBR = 3. Pixel size is 0.5 nm. e Configuration of a simulated dual-color dye-labelled origami for simulation of dual-color 2p-MINFLUX. Angles and distances are same with (a). f Simulation of dual-color 2p-MINFLUX. Simulation was repeated for 100 times for each dye, with N = 250, L = 50 nm, and SBR = 3. Scale bar: b–d, and f 5 nm