Fig. 5

Computational volumetric CRS imaging. a Setup of wide-field CARS holography. b From top left to bottom right, raw CARS hologram, amplitude, phase and digitally propagated CARS field at three different depths (z = 7.92 µm, 2.92 µm and -15.6 µm) of polystyrene microbeads in water. c Schematic of stimulated Raman projection (SRP) tomography with a rotational stage. SRP volumetric imaging of a single adipose cell is demonstrated. d tilt-angle stimulated Raman projection tomography (TSRPT). Tilt-angle beams incident at four different angles are focused by the objective. Volumetric reconstruction from four tilt-angle SRS projection images of PS beads is shown. e Principles of stimulated Raman scattering tomography (SRST) with frequency encoding. f SRST and conventional SRS imaging of mouse ear skin at different depth sections are illustrated. a, b are from reference [78], c, d are from references [80] and [81], respectively, e, f are from reference [85]