Fig. 3

Hyperspectral CRS image analysis methods. a Maximum intensity projection of a hyperspectral CH SRS image on mammalian cells. b Phasor plot of the hyperspectral image. Yellow boxes indicate manual segmentation. c Color-coded maps of cellular components based on phasor segmentation. d Flowchart of hyperspectral CRS image unmixing using MCR-ALS. e Concentration maps and f spectra after MCR-ALS analysis of a hyperspectral SRS image on Caenorhabditis elegans in the fingerprint region. LROs, lysosome-related organelles. a–c are from reference [55], d is from reference [21] and e–f are from reference [58]