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Fig. 3 | eLight

Fig. 3

From: Upconversion nanoparticles for super-resolution quantification of single small extracellular vesicles

Fig. 3

Co-localization of the large, medium and small EpCAM-mGFP EVs specifically labelled by UCNPs. a Schematic illustration of co-localization experiment. b, c, d, mGFP/ UCNPs double labelled EpCAM+EVs with the sizes decreasing from large to small under the 488 nm and 980 nm excitation of a purpose-built wide-field fluorescence microscope, confirming a relatively high degree of co-localization. Scale bar, 10 μm. f Pearson’s R analysis of mGFP-UCNPs co-localize for aggregated EVs (b). g Pearson’s R analysis of mGFP-UCNPs co-localize for large EVs (c)

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