Fig. 4From: A Through-Intact-Skull (TIS) chronic window technique for cortical structure and function observation in miceTIS window for long-term cortical blood flow monitoring. a Representative cortical vascular images from two-photon imaging at various depths on days 0 (the day TIS window was established), 7, and 10 after the TIS window establishment. The axial step was set at 2 μm. b Representative two-photon line-scanned images of blood vessel speed measurements at various depths on days 0 and 14 after the TIS window establishment (red numbers in the depth images indicate the line scan positions corresponding to the line scanned images to the right). The excitation wavelength was 920 nm (80 MHz, 140 fs). Similar results were obtained in 4 miceBack to article page