Fig. 4
From: Light-driven single-cell rotational adhesion frequency assay
Investigation of adhesion strength of single yeast cells using scRAFA. a Fraction of tracked cells with a total of 40 cells that show continuous rotation, transient adhesion and direct adhesion over a tracking period of 60 s. ConA + D-mannose: The substrate is functionalized with ConA and excess D-mannose is added into the solution. ConA: The substrate is functionalized with ConA and no D-mannose is added into the solution. b Fraction of transient ConA-mannoside adhesion events with lifetime \(\tau\) a ≥ t was plotted versus t for two strains of yeast cells, i.e., S. Cerevisiae and C. Albicans. Qa (\(\tau\) a ≥ t): fraction of transient adhesion events with lifetime \(\tau\) a ≥ t. t ranges from 0 to the maximum lifetime c Fraction of tracked cells with a total of 40 cells that show continuous rotation, transient adhesion, and direct adhesion over a tracking period of 60 s. WGA + GlcNAc: The substrate is functionalized with WGA and excess GlcNAc is added into the solution. WGA: The substrate is functionalized with WGA and no GlcNAc is added into the solution. d Fraction of transient WGA-chitin adhesion events with lifetime \(\tau\) a ≥ t was plotted versus t for two strains of yeast cells, i.e., S. Cerevisiae and C. Albicans. Qa (\(\tau\) a ≥ t): fraction of transient adhesion events with lifetime \(\tau\) a ≥ t. t ranges from 0 to the maximum lifetime