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Fig. 2 | eLight

Fig. 2

From: Two-photon MINFLUX with doubled localization precision

Fig. 2

Dependence of CRB on different parameters. a Dependence of CRB with respect to SBR and excitation wavelength. CRB is calculated with given L = 50 nm and N = 100. b Dependence of CRB with respect to SBR with fixed L. In the shaded area where (median) SBR \(\le\) 1, CRB increases (precision decreases) sharply. c, d Combined influence on CRB by SBR and L. SBR is given at \(L_0\) = 50 nm (blue lines). SBR decreases as L decreases (d), sets a lower bound for L (c). At optimal CRB (solid dots), \(SBR_{1p}\) \(\approx\) 0.81, \(SBR_{2p}\) \(\approx\) 2.36. Note the logarithmic scale for SBR (d). CRB is calculated with N = 100 for (b–d)

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